Fig. 7

Down regulation of CSN3 alters NF-κB-mediated signaling. a Immunofluorescence images of cells labeled with NF-κB p65 (Green) and Dapi (Blue). b The shNT-control and CSN3 knockdowns were grown to confluency, and differentiated for 48 h. Nuclear and cytoplasmic fractions were obtained and analyzed by immunoblot. c Quantification of nuclear NF-κB p65 expression normalized to TATA binding protein (TBP). * ** significantly different from shNT-control, # # # significantly deviated from shCSN3-Med group as analyzed by two-way ANOVA. *** and # # # p <0.001. d Quantification of IκB expression normalized to tubulin. Representative immunoblots are shown from samples run on a single gel for each antibody