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Table 1 Mean and standard deviation of apoptotic MCF-7 cells after 4 and 24 h exposure at three concentrations of compound 1 (11,13-diidrozaluzanin C) and 2 (Gochnatiolide C) associated with doxorubicin

From: Actions of sesquiterpene lactones isolated from Moquiniastrum polymorphum subsp. floccosum in MCF7 cell line and their potentiating action on doxorubicin

Concentrations Apoptosis Assay Comparison between 4 and 24 h
Mean of apoptotic cells ± SD (4 h) Mean of apoptotic cells ± SD (24 h)
Control 4.00 ± 1.87a 10.75 ± 2.86a p > 0,05
Doxorrubicin (D) - 0.3 μM 33.25 ± 5.34b 61.75 ± 10.82b *
11,13-diidrozaluzanin C (Compound 1)
Compound 1 – 14 μM 16.00 ± 1.87c 23.25 ± 3.83b *
Compound 1–28 μM 24.25 ± 5.71b,c 33.5 ± 3.77c p > 0,05
Compound 1–56 μM 21.5 ± 4.83b,c 17.5 ± 2.95a p > 0,05
Compound 1–14 μM + D 0.3 μM 47.00 ± 6.07b 47.25 ± 5.21b p > 0,05
Compound1 - 28 μM+ D 0.3 μM 39.75 ± 8.19b 51.25 ± 10.2b p > 0,05
Compound1 - 56 μM + D 0.3 μM 52.25 ± 9.8b 31.5 ± 4.6c *
Gochnatiolide C (Compound 2)
Compound 2–0.5 μM 24.50 ± 2.69b 40.00 ± 4.94c *
Compound 2–1.0 μM 41.75 ± 10.05b,c 51.50 ± 7.69b p > 0,05
Compound 2–2.0 μM 53.25 ± 6.13c 60.25 ± 10.03b p > 0,05
Compound 2–0.5 μM + D 0.3 M 31.25 ± 4.20b 31.25 ± 5.76c p > 0,05
Compound 2–1.0 μM + D 0.3 μM 40.00 ± 5.43b 45.25 ± 4.14c p > 0,05
Compound 2–2.0 μM + D 0.3 μM 46.25 ± 4.44b,c 62.00 ± 7.51b *
  1. Frequency of apoptotic cells after 4 and 24 h of test - free medium. SD: Standard Deviation. Compound 1 at concentrations of 14; 28 and 56 μM Compound 2 at concentrations of 0.5; 1.0 and 2.0 μM combined or not with doxorubicin IC 50 in MCF7 breast cancer cell line. We analyzed 100 cells, the data represent mean and standard deviation of the average of two independent replicates with two replicates. Different letters indicate statistically significant differences. The results were evaluated by NPAR1WAY/Wilkoxon. Significance * p <0.05