Fig. 2
The effects of E2 exposure on lomustine and vincristine cytotoxicity in D283 Med cells. Initial range finding concentration response analysis of the cytotoxic effects of a lomustine (IC50 = 12 μM) and b vincristine (IC50 = 1.5 nM) on D283 Med in a clonogenic assay of cell survival. c Quantification of surviving colony numbers from clonogenic assays of D283 Med cells exposed to 10, 20, or 40 μM lomustine with or without 10 nM E2 (n = 6 for each group). d Quantification of colony number from clonogenic assays of D283 Med cells exposed to 5, 10, or 20 nM vincristine with or without 10 nM E2 (n = 8 for each group). All results are expressed as mean ± SEM. Significant differences from the control group were determined by two-way ANOVA followed by Holm-Sidak’s post-test analysis and is indicated above the error bars; * p ≤ .05