Fig. 4
The effects of E2 exposure on cisplatin cytotoxicity in PFSK-1 CNS-PNET cells and Daoy cells. a Quantification of surviving colony numbers from clonogenic assays of PFSK-1 cells exposed to 2, 4 or 9 μM cisplatin with or without 10 nM E2 (n = 8 for each cisplatin treatment group except 4 μM where n = 4). b Quantification of colony number from clonogenic assays of PFSK-1 cells cotreated with 4 μM cisplatin and either vehicle, 10 nM E2, 10 nM fulvestrant (ICI), 10 nM E2 and 10 nM fulvestrant (ICI/E2), n = 4 for each group. c Quantification of surviving colony numbers from clonogenic assays of Daoy cells exposed to 2, 4 or 9 μM cisplatin with or without 10 nM E2. For each group n = 4. d Quantification of colony number from clonogenic assays of Daoy cells cotreated with 4 μM cisplatin and either vehicle, 10 nM E2, 10 nM fulvestrant (ICI), 10 nM E2 and 10 nM fulvestrant (ICI/E2), n = 8 for each group. e Analysis of the effects of 10 nM E2 on viability of Daoy cells. At T0 3000 cells were plated into 60 mm cell culture dishes, in growth media cells containing 10% CSS. At each indicated time point (hours post treatment) cells were harvested and trypan-excluding cells were counted. Vehicle was 0.0001% DMSO and replacement of CSS with 10% FBS served as a positive control. At each time point n = 10 for all treatments. Results are expressed as mean ± SEM. Significant differences from vehicle control are indicated above the treatment group error bars with individual comparisons indicated above brackets: * p ≤ .05; ** p ≤ 0.01; *** p ≤ .001; NS, not significant