Table 2 Primers sequences used in direct qMSP
MiRNA gene | Methylated primers | Unmethylated primers | Temperature (°C) | Amplicon size (bp) | ||
|---|---|---|---|---|---|---|
M | U | M | U | |||
miRNA-122 | F:5′-TTGTTTTGAAAATTATTTTTTTGTTC-3′ | F:5′-TTTTGAAAATTATTTTTTTGTTTGA-3′ | 57.2 | 57.3 | 122 | 120 |
R:5′-CATCTACTCACCTAATCCACGAT-3′ | R:5′-CCATCTACTCACCTAATCCACAAT-3′ | 56.5 | 58.5 | |||
miRNA-125b | F:5′-CGGTTAAAGTATAAATTATAGAGTTACGG-3′ | F:5′-TGGTTAAAGTATAAATTATAGAGTTATGG-3′ | 57.8 | 57.3 | 181 | 179 |
R:5′-ACTAACTACAAAACTTCCAAAAACG-3′ | R:5′-TAACTACAAAACTTCCAAAAACACC-3′ | 54.7 | 56.9 | |||
miRNA-106b | F:5’-TCGGAAATTTATTTGGAAGTTTATC-3’ | F:5′-TGGAAATTTATTTGGAAGTTTATTG-3’ | 59.2 | 53.9 | 105 | 105 |
R:5’-GACAATCTACTTCAACTCCTCGAC-3’ | R:5’-CAACAATCTACTTCAACTCCTCAAC-3’ | 57.2 | 57.7 | |||