Fig. 3
From: Sulforaphane attenuates pulmonary fibrosis by inhibiting the epithelial-mesenchymal transition
The effect of SFN on TGF-β/SMAD signalling in A549 epithelial cells and MRC-5 fibroblast cells. The cells were pre-treated for 1 h with the indicated concentrations of SFN and for 24 h with TGF-β1 (1.0 or 5.0 ng/ml). Western blotting for phosphorylated and total form of SMAD 2/3 was performed using the cell lysates of A549 epithelial cells (a) and MRC-5 cells (b). The data are expressed as means ± standard deviation of at least three different experiments