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Fig. 4 | BMC Pharmacology and Toxicology

Fig. 4

From: Mineral trioxide aggregate affects cell viability and induces apoptosis of stem cells from human exfoliated deciduous teeth

Fig. 4

Images of SHED cells with fragmented DNA marked using a TUNEL assay (In situ cell death detection kit, AP, Roche) at 2 days incubated with direct or indirect MTA contact. Positive cells show a bright green nucleus. DAPI staining was used to check the location of the nucleus. Apoptosis was indicated by TUNEL-positive cells. MTA_DC specimens showed positive staining, whereas the MTA_IDC specimen also showed positive staining, but not as obviously. White bar, 20µm

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