Fig. 4

Photomicrographs of the Hepa1c1c7 cells mitochondria labeled with rhodamine 123 (green) and nucleus with DAPI (blue) and analyzed by confocal laser microscopy. Cells were treated with PHO-S, DODAC and DODAC/PHO-S 1: 1, for a period of 6 h and mitochondria were labeled with rhodamine 123. a and b control; c and d PHO-S -0.3 mM; e and f PHO-S 2.0 mM; g and h DODAC/PHO-S 0.3 mM; i and j DODAC/PHO-S 2.0 mM; k and l DODAC 0.3 mM; m and n DODAC 2.0 mM. Scale bar = 10 and 20 μm