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Fig. 2 | BMC Pharmacology and Toxicology

Fig. 2

From: An inhibitor of 11-β hydroxysteroid dehydrogenase type 1 (PF915275) alleviates nonylphenol-induced hyperadrenalism and adiposity in rat and human cells

Fig. 2

11β-HSD1 and PPARα activity and expression in the liver from vehicle-, NP- and NP + PF-treated female rats. a: The 11β-HSD1 reductase activity was measured in the liver from vehicle-, NP- and NP + PF-treated female rats using 11-dehydrocorticosterone as a substrate in the presence NADPH. Values are shown as the means ± S.E.M. b and c: Expression of 11β-HSD1 (32 kDa) and PPARα (57 kDa) in livers from vehicle-, NP- and NP + PF-treated female rats were determined by western blot analysis. The representative immunoblot was shown in the upper part, and densitometry for 11β-HSD1 or PPARα normalized to GAPDH was shown in the bottom part. The values are presented as the means ± S.E.M. and are presented as the fold-change compared to the vehicle group. d and e: The mRNA levels of 11β-HSD1 and PPARα in the liver were determined by real-time PCR. The values are presented as the means ± S.E.M. and are presented as the fold-change relative to the vehicle group. +: p < 0.05, NP group compared with vehicle group. *, **: p < 0.05, 0.01, NP + PF group compared with the NP group

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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