Fig. 5
Effects of NP and PF on cell proliferation, cell viability and lipid accumulation. a: Human preadipocytes were seeded onto 96-well plates at a density of 4.5 × 103 cells/well and treated with NP combined with PF for 24 or 72 h. MTT reagent was added to the medium. After 4 h of incubation, the medium was aspirated, and 150 μl DMSO was added to each well. The absorbance was read at 570 nm. b: Human preadipocytes were incubated in differentiation medium (PADM) in the presence of NP combined with PF. After 6 days, MTT assay was performed to assess the cell viability. c: Human preadipocytes were incubated in differentiation medium (PADM) in the presence of NP combined with PF for 6 days. Oil Red O staining was performed, and stained lipids were extracted and quantified by measuring absorbance at 492 nm. Values are means of 3 independent experiments, are shown as the mean ± S.E.M, and are presented as the fold-change relative to control cells. a: cells incubated with PAM for 24 h; b and c: cells differentiated with only PADM for 6 days. +, +++: p < 0.05, 0.005, NP effect with the same concentration of PF. *: p < 0.05, PF effect with the same concentration of NP