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Fig. 7 | BMC Pharmacology and Toxicology

Fig. 7

From: An inhibitor of 11-β hydroxysteroid dehydrogenase type 1 (PF915275) alleviates nonylphenol-induced hyperadrenalism and adiposity in rat and human cells

Fig. 7

Effects of NP and PF on mRNA and protein expression levels by real-time PCR and western blot analysis. Human preadipocytes were incubated in differentiation medium (PADM) in the presence of NP (0 or 20 μM) combined with PF915275 (0, 2 or 4.5 μM). a, b and c: After 6 days of incubation, PPARγ, PPARα and FASN mRNA levels in the cells were determined by real-time PCR analysis, and the values were normalized against 18 s rRNA. d, e and f: After 6 days of incubation, PPARγ (57 kDa), PPARα (57 kDa) and FASN (273 kDa) protein levels in the cells were determined by western blot analysis. The representative immunoblot was shown in the upper part, and densitometry for PPARγ, PPARα or FASN normalized to GAPDH (for PPARγ, PPARα) or Vinculin (for FASN) was shown in the bottom part. The values, which are means of independent cultures, are presented as the means ± S.E.M. and represent the fold-change relative to control cells (cells differentiated with PADM only). +, ++: p < 0.05, 0.01, NP effect with the same concentration of PF. *, **: p < 0.05, 0.01, PF effect with the same concentration of NP

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