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Table 1 Real-time PCR primers

From: An inhibitor of 11-β hydroxysteroid dehydrogenase type 1 (PF915275) alleviates nonylphenol-induced hyperadrenalism and adiposity in rat and human cells

Gene

Sequence (5′ to 3′ forward)

Sequence (5′ to 3′ reverse)

11β-HSD1

CCTCCCCGTCCTGGTGCTCT

TGCTCCGAGTTCAAGGCAGCG

GAPDH

TGTGAACGGATTTGGCCGTA;

GATGGTGATGGGTTTCCCGT

PPARα

GCTCTGAACATTGGCGTTCG

TCAGTCTTGGCTCGCCTCTA

(h) FASN

CGCGT GGCCGGCTACTCCTAC

CGGCTGCCACACGCTCCTCT

(h) PPARα

CAGAACAAGGAGGCGGAGGTC

TTCAGGTCCAAGTTTGCGAAGC

(h) PPARγ

AGGCGAGGGCGATCTTGACAG

GATGCGGATGGCCACCTCTTT

(h) 18 s rRNA

TGCCATGTCTAAGTACGCACG

TTGATAGGGCAGACGTTCGA

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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