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Table 1 Summary of the purification steps of the L-asparaginase produced by Streptomyces brollosae NEAE-115

From: Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Purification step Total protein content (mg) L-asparaginase activity Recovery (%) Purification fold
Total activity (U) Specific activity (U/mg protein)
Culture filtrate 3120.42 30538.87 9.786 100 1
(NH4)2SO4, post dialysis 158.86 10,962 69.004 35.895 7.051
Ion exchange on DEAE Sepharose 10.371 795.152 76.671 7.254 7.835