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Table 5 Biochemical properties of some microbial L-asparaginases

From: Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Microbial source pH optima Temperature optima (°C) Km (M) Vmax Specific activity (U/mg protein) Molecular weight (kDa) References
Streptomyces albidoflavus 7.5 35 437 112 [29]
Streptomyces gulbargensis 9 40 2053 85 [13]
Streptomyces radiopugnans MS 6 40 5.98 × 10−2 3.547 IU/μg 5035.28 133.25 [36]
Enterobacter cloacae 7–8 35–40 1.58 × 10−3 2.22 IU/μg 105.07 52 [37]
Pseudomonas aeruginosa 9 37 0.147 × 10− 3 35.7 IU 1900 160 [30]
Vibrio cholerae 7 37 1.1 × 10− 3 1006 μM/min 648.9 132 [21]
Pseudomonas stutzeri 9 37 1.45 × 10−4 732.3 34 [38]
Pseudomonas fluorescens 8–9   4.1 × 10− 4 500 70 [39]
Azotobacter vinelandii 8.6 48 1.1 × 10− 4   2.47 84 [40]
E. coli 7–8 37 3.5 × 10− 3 150–250 139 [41,42,43]
Erwinia aroideae 8.2 45 2.8 × 10− 3 155 [44]
Corynebacterium glutamicum 7 40 2.5 × 10− 3 2020 80 [45]
Bacillus coagulans 8.8–9.7 55 5 × 10− 3 5.83 × 10− 5 M/h 10.9 84 [46]
Bacillus licheniformis 6–10 40 1.4 × 10−5 4.03 IU 697.09 134.8 [47]
Aspergillus oryzae CCT 3940 8 50 0.66 × 10−3 313 IU/mL 91 115 [48]
Fusarium culmorum ASP-87 8 45 3.57 × 10−3 0.5 μmol/mL/min 16.66 90 [22]