Fig. 5

ART inhibits liver fibrosis via the FAK/Akt/β-catenin pathway. a The relative viability of LX-2 cells was determined using the CCK-8 assay after treatment with different concentrations of the FAK inhibitor PF562271 (0, 1, 3, 5 and 7 μmol/l) for 12, 24, 48 or 72 h (n = 6); b The cells were treated with 3 μM PF562271 alone, 25 μg/ml ART alone, or the combination of 25 μg/ml ART and 3 μM PF562271 for 24 h, then harvested. The levels of FAK, p-FAK, Akt, p-Akt, GSK-3β, p-GSK-3β and β-catenin were measured using western blotting (n = 4). Each column shows the mean ± SEM. ^* means P < 0.05 vs control. ^** means P < 0.01 vs control. ^& means P < 0.05 vs ART group