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Table 4 Interference Studies of Structurally Related Compound

From: Modified enzyme multiplied immunoassay technique of methotrexate assay to improve sensitivity and reduce cost

Estimated MTX (μmoL/L) Estimated 7-hydroxy-methotrexate (μmoL/L) Measured MTX (n = 5) (μmoL/L) Bias %Bias
0.05 0.00 0.049   
0.05 0.506 0.0008 1.61
0.50 0.522 0.0024 4.82
1.00 0.543 0.0045 9.04
2.50 0.566 0.0068 13.65
5.00 0.597 0.0099 19.88