Table 2 Open scratch area of U2OS cells treated with putative migration inhibitors. The scratch area at time 0 was set 100%; *** p < 0.001, ** p < 0.01, * p < 0.05; n = number of repetitions. / = cells detached from flask surface

Paclitaxel

Control

41.03 ± 27.83

11.91 ± 14.40

6.09 ± 8.54

6

10,000

75.67 ± 28.21

70.15 ± 25.93

***

69.49 ± 25.96

***

6

2000

64.68 ± 20.47

57.75 ± 17.49

***

61.11 ± 17.56

***

6

400

59.24 ± 16.44

53.24 ± 14.03

***

54.84 ± 17.46

***

6

80

58.47 ± 13.84

50.23 ± 13.39

***

49.62 ± 14.53

***

6

16

48.24 ± 12.56

30.49 ± 18.99

26.34 ± 18.47

*

6

1.6

42.11 ± 27.84

15.68 ± 11.85

4.79 ± 5.37

4

0.16

42.37 ± 19.51

12.86 ± 7.46

2.89 ± 4.23

3

Vinblastine

Control

43.80 ± 32.59

10.22 ± 13.33

4.35 ± 6.95

4

5500

72.66 ± 19.76

57.06 ± 15.12

**

70.21 ± 14.25

***

4

1100

65.92 ± 31.03

58.11 ± 29.74

*

62.65 ± 36.28

*

4

220

67.60 ± 27.12

60.92 ± 23.69

**

61.14 ± 22.50

**

4

44

63.52 ± 28.10

55.48 ± 30.19

*

50.97 ± 33.90

*

4

8.8

69.83 ± 23.52

57.55 ± 30.94

*

55.31 ± 32.09

*

4

0.88

60.74

25.08

21.46

2

0.088

30.21

4.30

1.44

2

Colchicine

Control

48.05 ± 24.45

14.30 ± 14.71

7.31 ± 8.94

5

10,000

68.25 ± 24.54

61.62 ± 34.50

*

63.70 ± 33.17

**

5

2000

60.03 ± 25.98

47.87 ± 23.46

*

51.51 ± 32.26

*

5

400

51.41 ± 25.58

45.61 ± 24.09

*

42.70 ± 27.24

*

5

80

57.52 ± 23.55

52.54 ± 21.60

*

49.97 ± 27.97

*

5

16

48.62 ± 25.82

37.68 ± 25.16

37.71 ± 26.11

*

5

Compound

Concentration (nM)

24 h

48 h

72 h

n

Podophyllo-

toxin

Control

11.66 ± 3.82

2.22 ± 2.36

0.43 ± 0.75

3

10,000

65.10 ± 4.24

***

76.78 ± 1.37

***

71.37 ± 5.90

***

3

2000

70.81 ± 28.15

*

66.87 ± 15.93

**

84.08 ± 18.24

**

3

400

58.66 ± 6.67

***

60.56 ± 2.13

***

63.58 ± 12.85

**

3

80

66.88 ± 6.17

***

62.97 ± 5.34

***

79.59 ± 14.17

***

3

16

42.41 ± 1.51

***

34.57 ± 10.57

**

52.73 ± 8.81

***

3

3.2

10.45 ± 14.19

0

0

3

0.64

15.70 ± 22.11

0

0

3

Sanguinarine

Control

39.69 ± 13.67

10.73 ± 10.21

4.19 ± 7.67

4

5000

/

/

/

5

1000

39.62 ± 12.17

19.70 ± 5.54

13.10 ± 5.47

5

200

35.96 ± 18.07

18.12 ± 20.85

13.96 ± 20.04

5

40

29.66 ± 7.64

6.43 ± 7.75

4.37 ± 6.48

5

8

28.96 ± 15.04

5.07 ± 7.42

2.39 ± 4.84

5

Chelerythrine

Control

39.69 ± 13.67

10.73 ± 10.21

4.19 ± 7.67

4

12,000

84.35 ± 23.35

*

/

/

5

2400

34.43 ± 12.35

10.83 ± 14.73

9.34 ± 16.31

5

480

42.79 ± 13.29

15.89 ± 19.22

10.02 ± 18.69

5

96

29.69 ± 18.00

11.70 ± 21.10

9.28 ± 19.95

5

19.5

30.33 ± 17.73

9.07 ± 18.47

7.64 ± 16.94

5

Chelidonine

Control

44.80 ± 16.45

20.16 ± 22.85

14.38 ± 23.73

5

20,000

57.98 ± 11.72

43.41 ± 24.82

44.06 ± 30.05

5

4000

44.59 ± 15.78

25.26 ± 23.38

24.46 ± 31.62

5

800

45.80 ± 17.05

23.94 ± 23.20

21.03 ± 24.67

5

160

39.21 ± 12.32

15.26 ± 15.19

12.04 ± 16.10

5

32

30.50 ± 14.01

7.77 ± 11.29

8.22 ± 16.72

5

Homoharring-

tonine

Control

3.76 ± 2.83

0.31 ± 0.69

0.26 ± 0.58

3

8000

78.98 ± 12.39

***

/

/

3

1600

74.23 ± 20.66

**

68.25 ± 15.17

**

/

3

320

70.09 ± 6.42

***

72.37 ± 7.95

***

75.60 ± 7.17

***

3

64

85.25 ± 16.02

***

86.73 ± 12.13

***

83.94 ± 12.26

***

3

12.8

39.11 ± 7.84

**

28.99 ± 6.44

**

29.76 ± 7.19

**

3

2.56

13.45 ± 5.53

2.68 ± 3.97

1.95 ± 3.38

3

0.5

19.08 ± 5.65

*

4.31 ± 1.71

3.95 ± 3.51

3

0.1

2.18 ± 0.85

0

0

3

Doxorubicin

Control

30.29 ± 15.09

5.06 ± 4.76

0.36 ± 0.62

3

20,000

86.77 ± 11.70

**

94.33 ± 5.34

***

/

3

10,000

71.22 ± 4.93

*

76.25 ± 6.40

***

73.47 ± 4.82

***

3

5000

31.19 ± 2.67

11.32 ± 6.42

7.27 ± 4.59

3

2500

27.93 ± 17.02

6.43 ± 7.73

0.65 ± 1.12

3

1250

21.82 ± 17.07

4.76 ± 6.55

0.58 ± 1.01

3