Fig. 4

Activation of Wnt/β-catenin signaling to protect osteoblasts. a, the apoptosis of OB cells were analyzed by flow cytometry after olanzapine (40 μM), risperidone (40 μM), amisulpride (30 μM), aripiprazole (12 μM) with or without resveratrol (Res, 50 μM) treatment at 24 h. Bar graph indicates the percent of Annexin V-positive cells (apoptotic cells). b, β-catenin protein expression was measured by WB after olanzapine (40 μM), risperidone (40 μM), amisulpride (30 μM), aripiprazole (12 μM) with or without resveratrol (50 μM) treatment at 24 h in OB cells. The data were calculated with GraphPad Prism. c, Correlation between OB cells apoptosis rate and β-catenin protein expression in OB cells. *P < 0.05; **P < 0.01