Fig. 4

The detection of ROS generation using CellROX Green Reagent. In cells treated with DOX there was a high fluorescent signal deriving from mitochondria. In the case of A cultures the signal came from the nuclei. In DOX A, the signal came only from the nuclei. H showed oxidative signal in nuclei, however DOX and H combination – in both nuclei and mitochondria. The results present one representative experiment of three independently performed that showed similar patterns. HepG2 cells morphology was analysed under a phase-contrast microscope Nikon Eclipse Ti, magnification x300, scale bar = 100 μm. C – control, DOX – 1 μM doxorubicin, A – 100 μM apigenin, H – 200 μM hesperidin, DOX A – 1 μM doxorubicin and 100 μM apigenin, DOX H – 1 μM doxorubicin and 200 μM hesperidin