Fig. 4
From: Dexamethasone suppresses the differentiation of stem Leydig cells in rats in vitro
The expressions of Nr3c1 in the Leydig cell lineage and the effects of DEX on Leydig cell gene expressions. Stem (SLC), progenitor (PLC), immature (ILC), adult (ALC) Leydig cells were isolated from 6-, 21-, 35-, and 90-day-old rat testes, respectively. Seminiferous tubules were cultured in the LIM in the presence of DEX alone or with RU38486 or RU38486 alone for 21 days, then the qPCR measured mRNA levels of Leydig cell-specific genes (n = 4–6). D10 = DEX 10 nM, D100 = DEX 100 nM, R = RU38486 1 μM. The data are presented as the mean ± SEM. Identical letters showed no significant difference between two groups at P < 0.05