Fig. 6

DEX does not affect stem Leydig cell proliferation. Panel a-c: EDU-labeled stem Leydig cells (green color, white arrow) afte treatment in the BM for 5 days. Panel: (a) Control; (b) DEX 100 nM; (c) EDU-labeled stem Leydig cell numbers (n = 6). Panel D: Medium testosterone (T) levels after seminiferous tubules were treated with various concentrations of DEX (0–100 nM) in the BM for 7 days and then these tubules were switched to the LIM for additional 14 days (n = 4–8). In Panel c and d, the data were presented as the mean ± SEM and there was no significant difference when compared to control group at P < 0.05