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Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: Influence of N-acetyl-L-cysteine against bisphenol a on the maturation of mouse oocytes and embryo development: in vitro study

Fig. 1

Effect of 50 μg/mL BPA on ROS levels and GSH content in mouse oocytes. a. Intracellular ROS levels in mouse oocytes in the control and treatment groups. Oocytes exposed to 50 μg/mL BPA showed higher dichlorodihydrofluorescein fluorescence than those in the control group. ROS levels are presented as the average fluorescence intensity value, which was calculated from the fluorescence intensity values of multiple oocytes. Each treatment was repeated 3–4 times, with each replicate including approximately 10 oocytes. b. Intracellular GSH content in mouse oocytes in the control and treatment groups. Oocytes in the 50 μg/mL BPA treatment group had a lower GSH content than those in the control group. Each treatment was repeated 3 times, with each replicate containing approximately 25 oocytes. The data were analysed with an independent-samples T test. a, bValues with different letters above the bars differ significantly (P < 0.05). C, control; NAC, 100 μM N-Acetyl-L-cysteine; BPA, 50 μg/mL bisphenol A; NAC + BPA, combined treatment with N-acetyl-L-cysteine (100 μM) and bisphenol A (50 μg/mL). The photographs in C and D show the fluorescence intensity in the control and BPA groups, respectively. Scale bar, 100 μm

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