Influence of N-acetyl-L-cysteine against bisphenol a on the maturation of mouse oocytes and embryo development: in vitro study

Table 2 Effect of BPA on subsequent embryo development after IVF

Bisphenol A Concentration(μg/mL)	ooctyes inseminated	oocytes fertilized (%)	4-cell rate (%)	Blastocysts rate (%)
(Control)0	105	86.7 ± 1.0^a	85.7 ± 1.4^a	64.8 ± 1.8^a
20	109	81.1 ± 1.4^a	84.4 ± 1.4^a	55.5 ± 1.9^b
50	107	64.6 ± 2.1^b	84.7 ± 2.0^a	44.3 ± 3.0^c
100	110	38.4 ± 3.0^c	83.4 ± 1.7^a	24.1 ± 3.4^d

After 14 h of maturation, the embryo development of oocytes following IVF was tested. BPA at 50 and 100 μg/mL significantly decreased the rates of fertilization and blastocyst formation. Each treatment was repeated 3 times, and each replicate contained 30–40 oocytes. The data were analysed with one-way ANOVA, followed by Dunnett’s post-hoc test. ^a-dValues with different superscripts in the same column differ significantly (P < 0.05)

ISSN: 2050-6511