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Fig. 5 | BMC Pharmacology and Toxicology

Fig. 5

From: Identification of undecylenic acid as EAG channel inhibitor using surface plasmon resonance-based screen of KCNH channels

Fig. 5

Undecylenic acid inhibits currents from mEAG channels. a Representative mEAG current traces recorded in the inside-out configuration in the absence (black) and presence (red) of 50 μM undecylenic acid. b The tail current recorded at − 100 mV after a voltage step to + 70 mV in the absence (black) and presence (red) of 50 μM undecylenic acid. The grey lines represent fits of the tail currents with a single exponential function with the time constants of deactivation of 3.1 ms in the absence and 2.1 ms in the presence of undecylenic acid. c Plots of the percent inhibition of tail currents versus the undecylenic acid concentration. Tail currents were recorded at − 100 mV after a voltage step to 70 mV. The line represents fit of the data with Hill equation with the IC50 of 1.06 ± 0.03 μM. d Plot of the averaged percent inhibition of tail currents versus voltage obtained in the presence of 50 μM undecylenic acid (n = 4). e Conductance-voltage relationship in the absence (black) and presence (red) of 50 μM undecylenic acid. The black and red lines represent fits of the conductance-voltage relationships in the absence (black) and presence (red) of 50 μM undecylenic acid with a Boltzmann function with V1/2 of − 30.9 ± 1.5 mV and − 30.3 ± 1.6 mV, respectively

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