Fig. 8From: Differential role of dose and environment in initiating and intensifying neurotoxicity caused by MDMA in ratsEffects of MDMA doses and environments, respectively, on initiating and intensifying serotonergic injury. Serotonergic axonal retrograde transportation was used to estimate those effects. a Timeline of MDMA or SAL treatment followed by Tcor measurement (day 1), fluorogold administration (FG; day 7) and tissue sectioning (day 14). b Immunohistochemical staining of tryptophan hydroxylase-2 (TpH2)-containing neurons in the DRN retrograded with FG. Photomicrographs show representative changes in FG-positive (FG+) or/and TpH2-positive (TpH2+) neurons in the DRN. The top row, the FG+ neurons; the middle row, the TpH2+ neurons; the bottom row, FG+/TpH2+. c Cell counts of FG+ and FG+/TpH2+ neurons (N = 4–6). **P < 0.01 vs. the SAL control, examined using unpaired t-test. CTL-S, saline as control; HD-H ─, high-dose-induced hypothermia; HD-H+, high-dose-induced hyperthermia; LD-H+, low-dose-induced hyperthermiaBack to article page