Fig. 1

Autophagy and neurotoxicity were triggered by CORT in PC12 cells. a Changes in the morphology and number of PC12 cells were observed by optical microscope (Scale bar, 25 μm). b PC12 cell viability was measured by MTT assay (n = 3). c PC12 cells lysates were subjected to Western blotting using LC3 and tubulin antibodies (n = 3). d Densitometric values of LC3-II and LC3-I were quantified using the AlphaEaseFC software. Data are presented as mean ± SD. ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 compared with the vehicle group. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the CORT group