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Fig. 5 | BMC Pharmacology and Toxicology

Fig. 5

From: Chlorogenic acid protects PC12 cells against corticosterone-induced neurotoxicity related to inhibition of autophagy and apoptosis

Fig. 5

The inhibition of autophagy by CGA protected against CORT-induced apoptosis. a PC12 cells were incubated with or without CORT in the presence of CGA or autophagy inhibitor 3-MA for 24 h. Changes in the expression of LC3 were examined by Western blot (n = 3). b PC12 cells were incubated with or without CORT in the presence of CGA or autophagy inhibitor 3-MA for 24 h. Changes in the expression of C-PARP were examined by Western blot (n = 3). c Densitometric values of LC3-II and LC3-I were quantified using the AlphaEaseFC software. d Densitometric values of C-PARP were quantified using the AlphaEaseFC software. e PC12 cells were incubated with or without CORT in the presence of CGA or autophagy inhibitor CQ for 24 h. Changes in the expression of LC3 were examined by Western blot (n = 3). f PC12 cells were incubated with or without CORT in the presence of CGA or autophagy inhibitor CQ for 24 h. Changes in the expression of C-PARP were examined by Western blot (n = 3). g Densitometric values of LC3-II and LC3-I were quantified using the AlphaEaseFC software. h Densitometric values of C-PARP were quantified using the AlphaEaseFC software. Data are presented as mean ± SD. # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the vehicle group.* P < 0.05, ** P < 0.01, *** P < 0.001 compared with the CORT group

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