Fig. 2
From: SBF-1 inhibits contact hypersensitivity in mice through down-regulation of T-cell-mediated responses
SBF-1 suppressed T cell activation through inhibiting AKT signaling. T cells were isolated from lymph node of BALB/c mice and then incubated with SBF-1 in the presence of 5 μg/ml Con A for 24 h at 37 °C. a–b Cells were collected and analyzed for CD69 and CD25 expressions by flow cytometry. Data a shown here are one of three different experiments with similar results. Data b are mean ± SEM of three independent experiments. P values were determined by one-way ANOVA with Tukey’s correction. *P < 0.05, **P < 0.01. c Cells were harvested and the whole cell extracts were analyzed by western blotting for AKT and the phosphorylation of AKT. Data shown here are one of three different experiments with similar results