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Table 2 Effect of median lethal concentrations of flubendiamide and copper alone and in the presence of different concentrations of resveratrol, catechin, curcumin and α-tocopherol on viability, micronuclei and comet formation in rat splenocytes following their in vitro exposure

From: In vitro and in vivo effects of flubendiamide and copper on cyto-genotoxicity, oxidative stress and spleen histology of rats and its modulation by resveratrol, catechin, curcumin and α-tocopherol

TreatmentsaNonviable cells (%)aMicronuclei (%)No. of Comet/ 100 cells (%)
Control5.41 ± 0.330.96 ± 0.083.09 ± 0.31
DMSO (50 μl)8.59 ± 0.881.36 ± 0.084.58 ± 0.28
Dexamethasone (20 μM)7.60 ± 0.2027.69 ± 0.87
Flubendiamide (40 μM)71.88 ± 2.907.86 ± 0.1722.90 ± 0.90
Resveratrol (5 μM) + Flubendiamide (40 μM)50.00 ± 1.851.20 ± 0.1720.15 ± 1.91
Resveratrol (10 μM) + Flubendiamide (40 μM)24.36 ± 0.881.10 ± 0.0515.44 ± 1.47
Catechin (10 μM) + Flubendiamide (40 μM)53.66 ± 1.761.43 ± 0.2414.80 ± 1.25
Catechin (20 μM) + Flubendiamide (40 μM)52.46 ± 2.331.33 ± 0.0812.64 ± 0.57
Curcumin (5 μM) + Flubendiamide (40 μM)56.25 ± 3.053.13 ± 0.127.58 ± 0.89
Curcumin (10 μM) + Flubendiamide (40 μM)38.24 ± 3.181.40 ± 0.157.20 ± 0.32
α-tocopherol (5 μM) + Flubendiamide (40 μM)55.00 ± 0.332.10 ± 0.4011.56 ± 0.33
α-tocopherol (10 μM) + Flubendiamide (40 μM)40.26 ± 2.021.93 ± 0.296.96 ± 0.30
α-tocopherol (20 μM) + Flubendiamide (40 μM)17.65 ± 0.573.30 ± 0.264.89 ± 0.33
Copper (40 μM)81.11 ± 6.069.16 ± 0.2129.59 ± 1.76
Resveratrol (5 μM) + Copper (40 μM)76.54 ± 4.844.80 ± 0.2025.33 ± 0.47
Resveratrol (10 μM) + Copper (40 μM)30.43 ± 4.041.90 ± 0.329.69 ± 0.66
Catechin (10 μM) + Copper (40 μM)72.13 ± 3.715.20 ± 0.2015.12 ± 0.32
Catechin (20 μM) + Copper (40 μM)65.82 ± 1.412.10 ± 0.3612.41 ± 1.20
Curcumin (5 μM) + Copper (40 μM)64.18 ± 3.843.47 ± 0.1416.80 ± 0.87
Curcumin (10 μM) + Copper (40 μM)59.68 ± 4.332.63 ± 0.2112.71 ± 1.32
α-tocopherol (5 μM) + Copper (40 μM)59.72 ± 5.03.33 ± 0.2410.33 ± 1.20
α-tocopherol(10 μM) + Copper (40 μM)54.73 ± 4.911.93 ± 0.1815.20 ± 1.45
α- tocopherol (20 μM) + Copper (40 μM)51.06 ± 4.183.16 ± 0.2610.61 ± 0.66
  1. aData presented are Mean + SEM of three observations