Skip to main content

Table 3 Effect of oral administration of α-tocopherol (100 mg/kg) on certain splenic oxidative stress biomarkers following 90 days oral exposure of rats to copper (33 mg/kg) and flubendiamide (200 mg/kg) alone and both in combination (33 mg/kg + flubendiamide 200 mg/kg)

From: In vitro and in vivo effects of flubendiamide and copper on cyto-genotoxicity, oxidative stress and spleen histology of rats and its modulation by resveratrol, catechin, curcumin and α-tocopherol

GroupsTreatmentProtein in splenic tissue
(mg/ml)
LPO (nM MDA/g tissue)SODa
(U/ mg of protein)
Catalase
(mM H2O2 utilized/min
mg of protein)
GSH
(mM GSH/g tissue)
GSTa(μM of CDNB-GSH conjugate min-1 mg-1 protein)GPx
(nM NADPH utilized/min/mg protein)
IControl3.42 ± 0.56 a50.56 ± 1.60a3.93 ± 0.35a85.32 ± 7.35a2.38 ± 0.21abc1.62 ± 0.12a1.93 ± 0.35a
IIVehicle control (Corn oil)4.05 ± 1.02 a61.78 ± 2.12ab4.16 ± 0..36a79.07 ± 10.61a2.48 ± 0.17ab1.74 ± 0.14a0.96 ± 0.37ab
IIIα-tocopherol (100 mg/kg)3.21 ± 0.41 a68.56 ± 8.08ab3.33 ± 0.40ab87.94 ± 9.15a2.49 ± 0.08a1.66 ± 0.15a0.59 ± 0.06ab
IVCopper sulphate (33 mg/kg)2.59 ± 0.32 a81.65 ± 4.08b1.82 ± 0.25b80.54 ± 7.29a1.91 ± 0.04abc1.65 ± 0.20a0.49 ± 0.04b
VFlubendiamide (200 mg/kg)3.69 ± 0.61 a81.70 ± 5.57b3.40 ± 0.38a60.37 ± 7.99a1.84 ± 0.00c0.61 ± 0.12b0.37 ± 0.06 b
VICopper sulphate (33 mg/kg) + Flubendiamide (200 mg/kg)2.30 ± 0.34 a75.06 ± 7.67b3.27 ± 0.34ab98.76 ± 10.80a1.96 ± 0.12abc1.34 ± 0.25a0.43 ± 0.11b
VIICopper sulphate (33 mg/kg) + α-tocopherol (100 mg/kg)2.81 ± 0.37 a77.13 ± 10.74b3.02 ± 0.25ab83.48 ± 8.79a1.87 ± 0.09bc1.50 ± 0.09a1.16 ± 0.45ab
VIIIFlubendiamide (200 mg/kg) + α-tocopherol (100 mg/kg)4.16 ± 0.52 a61.14 ± 6.50ab4.42 ± 0.40a61.74 ± 10.55a2.19 ± 0.19abc1.43 ± 0.15a0.68 ± 0.36ab
IXCopper sulphate (33 mg/kg) + Flubendiamide (200 mg/kg)) + α-tocopherol (100 mg/kg)2.85 ± 0.27 a68.96 ± 4.84ab3.05 ± 0.37ab81.40 ± 7.19a2.24 ± 0.10abc1. 95 ± 0.30a1.09 ± 0.48ab
  1. • Values (Mean ± SEM; n = 6) bearing different superscripts in the same column differed significantly (P < 0.05)
  2. aValues are Mean ± SEM of five animals only