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Table 3 Effect of oral administration of α-tocopherol (100 mg/kg) on certain splenic oxidative stress biomarkers following 90 days oral exposure of rats to copper (33 mg/kg) and flubendiamide (200 mg/kg) alone and both in combination (33 mg/kg + flubendiamide 200 mg/kg)

From: In vitro and in vivo effects of flubendiamide and copper on cyto-genotoxicity, oxidative stress and spleen histology of rats and its modulation by resveratrol, catechin, curcumin and α-tocopherol

Groups

Treatment

Protein in splenic tissue

(mg/ml)

LPO (nM MDA/g tissue)

SODa

(U/ mg of protein)

Catalase

(mM H2O2 utilized/min

mg of protein)

GSH

(mM GSH/g tissue)

GSTa(μM of CDNB-GSH conjugate min-1 mg-1 protein)

GPx

(nM NADPH utilized/min/mg protein)

I

Control

3.42 ± 0.56 a

50.56 ± 1.60a

3.93 ± 0.35a

85.32 ± 7.35a

2.38 ± 0.21abc

1.62 ± 0.12a

1.93 ± 0.35a

II

Vehicle control (Corn oil)

4.05 ± 1.02 a

61.78 ± 2.12ab

4.16 ± 0..36a

79.07 ± 10.61a

2.48 ± 0.17ab

1.74 ± 0.14a

0.96 ± 0.37ab

III

α-tocopherol (100 mg/kg)

3.21 ± 0.41 a

68.56 ± 8.08ab

3.33 ± 0.40ab

87.94 ± 9.15a

2.49 ± 0.08a

1.66 ± 0.15a

0.59 ± 0.06ab

IV

Copper sulphate (33 mg/kg)

2.59 ± 0.32 a

81.65 ± 4.08b

1.82 ± 0.25b

80.54 ± 7.29a

1.91 ± 0.04abc

1.65 ± 0.20a

0.49 ± 0.04b

V

Flubendiamide (200 mg/kg)

3.69 ± 0.61 a

81.70 ± 5.57b

3.40 ± 0.38a

60.37 ± 7.99a

1.84 ± 0.00c

0.61 ± 0.12b

0.37 ± 0.06 b

VI

Copper sulphate (33 mg/kg) + Flubendiamide (200 mg/kg)

2.30 ± 0.34 a

75.06 ± 7.67b

3.27 ± 0.34ab

98.76 ± 10.80a

1.96 ± 0.12abc

1.34 ± 0.25a

0.43 ± 0.11b

VII

Copper sulphate (33 mg/kg) + α-tocopherol (100 mg/kg)

2.81 ± 0.37 a

77.13 ± 10.74b

3.02 ± 0.25ab

83.48 ± 8.79a

1.87 ± 0.09bc

1.50 ± 0.09a

1.16 ± 0.45ab

VIII

Flubendiamide (200 mg/kg) + α-tocopherol (100 mg/kg)

4.16 ± 0.52 a

61.14 ± 6.50ab

4.42 ± 0.40a

61.74 ± 10.55a

2.19 ± 0.19abc

1.43 ± 0.15a

0.68 ± 0.36ab

IX

Copper sulphate (33 mg/kg) + Flubendiamide (200 mg/kg)) + α-tocopherol (100 mg/kg)

2.85 ± 0.27 a

68.96 ± 4.84ab

3.05 ± 0.37ab

81.40 ± 7.19a

2.24 ± 0.10abc

1. 95 ± 0.30a

1.09 ± 0.48ab

  1. • Values (Mean ± SEM; n = 6) bearing different superscripts in the same column differed significantly (P < 0.05)
  2. aValues are Mean ± SEM of five animals only