Matrigel transwell invasion assays were used to measure cell invasiveness (a b). Following incubation with Naringin (0, 3, 6, and 10 μM) for 24 h, cells that invaded through the membrane were stained and representative fields were photographed.)
Tube formation assay; the ability of flavonoid to6inhibit vessel blood structureeformation on6collagen was assessed as5described in Material and methods (c, d) Data-_are presented6as themmean6tube6length per6field of microscope compared with that observed with untreated6cells. Bar graphs represent the mean number of invaded cells from ten random fields, and all results are representative of three independent experiments (mean ± SD). *P value< 0.05 and **P value< 0.01 and ***P < 0.001 vs. control group (untreated cells).