Fig. 2

Psoralen alleviated HG-induced viability decrease in HK-2 cells via upregulating miR-874. a HK-cells were treated with 10 μM psoralen for 48 h. The levels of miR-214, miR-379, miR-874, miR-770, miR-22 were examined by RT-qPCR. b HK-2 cells were exposure to 30 mM glucose and 10 μM psoralen for 48 h, the level of miR-874 was detected by RT-qPCR. c MiR-874 antagomir was transfected into HK-2 cells for 24 h, the level of miR-874 was quantified by RT-qPCR. d HK-2 cells were transfected with miR-874 antagomir for 24 h. Then, cells were exposure to 30 mM glucose and 10 μM psoralen for 48 h. Subsequently, CCK-8 was used to measure cell viability of all groups (HK-2, HG stimulated HK-2, HG+ psoralen treated HK-2, HG+ psoralen+ miR-874 antagomir treated HK-2). e, f Ki-67 staining was used to evaluate cell proliferation. **P < 0.01 compared with control group; ^##P < 0.01 compared with HG group; ^^P < 0.01 compared with HG + psoralen group