Fig. 3

Psoralen alleviated HG-induced apoptosis in HK-2 cells via upregulating miR-874. a, b HK-2 cells were transfected with miR-874 antagomir for 24 h. Then, 30 mM glucose and 10 μM psoralen was supplemented in medium for 48 h. Cell apoptosis of all groups were quantified by Annexin V/PI staining and flow cytometry (groups: natural HK-2, HG stimulated HK-2, HG+ psoralen treated HK-2, HG+ psoralen+ miR-874 antagomir treated HK-2). c-g The expressions of apoptosis related proteins were quantified by Western blot. β-actin was used as a loading control. **P < 0.01 compared with control group; ^##P < 0.01 compared with HG group; ^^P < 0.01 compared with HG + psoralen group