Fig. 6

Inhibition of lipolysis in adipocytes reverses adipocyte-conditioned medium-induced resistance of tumor cells to lapatinib. A) Adipocytes were exposed to different metabolic modulators. After 6 h, the supernatants were harvested and added to BT-474 cells prior to exposure to lapatinib. BT-474 were also exposed to control medium or adipocyte-conditioned medium (#3T3-CM). n ≥ 3. B) adipocytes were exposed to lipolysis stimulators or inhibitors. The supernatants were harvested and used as in A) and IC50 values were calculated for each condition. n ≥ 3. C) mRNA was extracted from adipocytes after exposure to terbutaline, salbutamol, dobutamine, isoprenaline, atenolol and propranolol and the expressions of the genes involved in lipolysis stimulation were evaluated and normalized on the expression of the genes in adipocytes cultured without treatment. n ≥ 3. ** p < 0,01