Fig. 2

Effects of tiotropium/olodaterol on CSE-induced cell death in BEAS-2B cells. a Cell viability of BEAS-2B cells after pretreatment with 25 μM tiotropium + 10 μM Olodaterol for 4 h, followed by CSE treatment for 24 h. b Cell viability of BEAS-2B cells after pretreatment with 25 μM tiotropium + 10 μM Olodaterol for 4 h, followed by CSE treatment for 24 h. Cell viability was determined using the MTT assay. The absorbance of the reaction solution at 570 nm was recorded. Data are presented as means ± SD from triplicate samples for each treatment. *P < 0.05 versus 5% CSE-treated group. Tio: tiotropium; Olo: Olodaterol