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Fig. 6 | BMC Pharmacology and Toxicology

Fig. 6

From: Tiotropium/Olodaterol treatment reduces cigarette smoke extract-induced cell death in BEAS-2B bronchial epithelial cells

Fig. 6

Effect of Tiotropium/olodaterol on CSE-mediated autophagy in BEAS-2B cells in short term treatment. a BEAS-2B cells were pretreated with tiotropium/olodaterol for 4 h, followed by treatment with or without 2.5 and 5% CSE for 6 h. AVO staining and flow cytometry analysis were then performed. AO accumulates and aggregates are characterized by yellow-orange fluorescence, and normal cells with AO are characterized by green fluorescence. b Western blot analysis of the expression of LC3 and Beclin 1 in BEAS-2B cells. *P < 0.05 versus 5% CSE-treated group. Tio: tiotropium; Olo: Olodaterol

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