Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Table 1 Qualitative phytochemical analysis of Symplocos racemosa bark

Phytoconstituents	Detected group	Stock solution (mg/mL)	Antimicrobial activity
Alkaloids		NA	NA
Mayer’s reagent test	- ^a
Hager’s reagent test	–
Wagner reagent test	–
Flavonoids		54.75	+++ ^c
Shinoda test (Magnesium turnings)	+^b
Zinc-hydrochloride reduction test	+
Lead acetate test	+
Ferric chloride reagent test	–
Saponins		41.6	+ ^d
Froth test	+
Tannins		100.5	+
Ferric chloride reagent test	+
Lead acetate test	+
Cardiac glycosides		59.5	++ ^e
Keller- killiani test	+
Terpenoids
Triterpenes (Salkowski’s test)	+	12	- ^f
Diterpenes (Copper acetate test)	–	NA	NA
Anthranol glycosides		ND	ND
Borntrager’s test	+
Phytosterols		38	–
Libermann Burchard’s test	+
Salkowski’s test	+
Coumarins	–	ND	–

^a- absent; ^b- present; ^c- most active; ^d- least active; ^e- active; ^f- not active
NA– Not applicable, ND– Not done

ISSN: 2050-6511