Fig. 6

Flow cytometry plots of control Huh7 cells and the groups treated with 5-FU, TPGS-FA, NC, and TPGS-FA/NC at a concentration of 50 μg/mL for 24 h. In vitro apoptosis was observed by propidium iodide (PI)/Annexin V-FITC dual staining and fluorescence-activated cell sorting (FACS) analysis (Q2 = Annexin V-FITC and PI positive, indicating cells in late apoptosis or already dead; Q3 = PI negative & Annexin V-FITC positive, indicating early apoptotic cells). Source data are provided as a Source Data file