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Fig. 2 | BMC Pharmacology and Toxicology

Fig. 2

From: Investigating the potential effects of selective histone deacetylase 6 inhibitor ACY1215 on infarct size in rats with cardiac ischemia-reperfusion injury

Fig. 2

The protocol of cardiac IR injury and administration of test compounds. Adult male Wistar rats (weighing 200–250 g) were intubated and ventilated using a small animal ventilator under anesthesia with intraperitoneal injection of pentobarbital sodium (50 mg/kg). After thoracotomy, the left anterior descending (LAD) coronary artery was ligated by 7–0 silk sutures. Each rat was subjected to ischemia for 45 min followed by reperfusion for 2 h with removal of silk sutures. The dosage of ACY1215 and MPT0E028 used in the present study were 50 mg/kg and 25 mg/kg, respectively. Rats were received treatment by intraperitoneal injection. HDAC inhibitors and vehicle were given within 5 min before reperfusion. After reperfusion for 2 h, rats were euthanized to collect blood and tissue samples for subsequent analysis. DMSO = dimethyl sulfoxide; IR = ischemia-reperfusion; MI = myocardial infarction

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