Skip to main content
Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: Depressive effectiveness of vigabatrin (γ-vinyl-GABA), an antiepileptic drug, in intermediate-conductance calcium-activated potassium channels in human glioma cells

Fig. 1

Effect of VGB on the activity of intermediate-conductance Ca2+-activated K+ (IKCa) channels expressed in human 13–06-MG glioma cells. In this set of experiments, 13–06-MG cells were bathed in normal Tyrode’s solution containing 1.8 mM CaCl2 and single-channel current recordings were made. The probability of IKCa channels opening was measured at − 80 mV relative to the bath. a Original current traces for IKCa channels obtained in the absence (left) and presence (right) of VGB (10 μM). Note that channel opening gives a downward deflection in current. b Original IKCa channel traces taken in the absence (upper) and presence (lower) of TRAM-34 (3 μM). c Summary of the data showing the effects of control, extracellular Ca2+ (0 mM), extracellular Ca2+ (3.6 mM), VGB, TRAM-34 (3 μM), and VGB (10 μM) plus DCEBIO (10 μM) on IKCa channel activity. The probability of IKCa channel opening was measured at − 80 mV relative to the bath. Each bar indicates the mean ± SEM (n=9–11). *Significantly different from control (i.e., in the presence of 1.8 mM Ca2+, but VGB was not present) (P< 0.05) and **significantly different from the VGB alone group (P< 0.05)

Back to article page