Fig. 5

Failure of VGB to modify the activity of K_IR channels in human 13–06-MG cells. In this set of experiments, we bathed cells in Ca²⁺-free Tyrode’s solution and, during the recording, we backfilled the pipette by using K⁺-containing solution. The activity of K_IR channels was detected at − 80 mV relative to the bath. a Single K_IR channels obtained in the absence (upper) and presence (lower) of 10 μM VGB. The downward deflection denotes the channel opening event. b Summary bar graph depicting the effect of VGB and VGB plus BaCl₂ on the activity of K_IR channels in human 13–06-MG cells (mean ± SEM; n=7 for each bar). ^*Significantly different from the control or 10 μM VGB alone (P< 0.05). VGB: 10 μM VGB; BaCl₂: 1 mM BaCl₂