Fig. 2
Inhibitory effect of bupivacaine on SK2 currents was reversible (n = 7 for each concentration).SK2 currents (0 mV) obtained at baseline and in inhibition and washout phases with exposure to 1μM, 10μM, and 100μM bupivacaine, respectively. Baseline: perfusion with Tyrode’s solution. Inhibition: perfusion with Tyrode’s solution containing bupivacaine. Washout: replacement of bupivacaine-containing Tyrode’s solution with normal Tyrode’s solution. Normalization inhibition was calculated as (Currentbaseline− Currentinhibition)/Currentbaseline. The intrapipette free calcium concentration was 1μM. *P < 0.05, compared with the baseline value