Fig. 3
Effect of calcium concentration on the inhibitory effect of bupivacaine. a SK2 currents obtained at 0 mV in the presence of different concentrations of free calcium. b Degree of inhibition of SK2 currents (0 mV) by 10μM bupivacaine when the pipette solution contained different concentrations of free calcium. Baseline: perfusion with Tyrode’s solution. Inhibition: perfusion with Tyrode’s solution containing bupivacaine. Washout: replacement of bupivacaine-containing Tyrode’s solution with normal Tyrode’s solution. Normalization inhibition was calculated as (Currentbaseline − Currentinhibition)/Currentbaseline. *P < 0.05, between 0.25μM group and 0.5μM group, #P < 0.05, between 0.5μM group and 1.0μM group. @P < 0.05, between inhibition value and baseline value