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Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: Gambogic acid protects LPS-induced apoptosis and inflammation in a cell model of neonatal pneumonia through the regulation of TrkA/Akt signaling pathway

Fig. 3

The effect of GA on TrkA/Akt in LPS-treated WI-38 cells. aWI-38 cells were cultured in vitro (Ctrl), or treated with 10mg/ml LPS for 24h (LPS), or pre-incubated with 50 nM GA for 24h prior to LPS treatment (LPS + GA). Western blot analysis was conducted to examine protein productions of TrkA, p-TrkA and Akt. bFor blot result in (A), TrkA protein expressions were quantitatively compared between WI-38 cells under Ctrl, LPS and LPS + GA conditions (∆ P > 0.05). cFor blot result in (A), phosphor-TrkA (p-TrkA) protein expressions were quantitatively compared between WI-38 cells under Ctrl, LPS and LPS + GA conditions (* P < 0.05). dFor blot result in (A), Akt protein expressions were quantitatively compared between WI-38 cells under Ctrl, LPS and LPS + GA conditions (* P < 0.05)

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