Table 2 Biochemical parameters of Sprague Dawley rats administration with subtilisin QK for 28 days and 28 days recovery

D₂₈

TBIL (umol/L)

0.3–1.2

1.04 ± 0.14

1.04 ± 0.23

1.25 ± 0.14*

1.22 ± 0.13

1.90 ± 0.27

1.94 ± 0.42

2.13 ± 0.35

2.19 ± 0.26

n = 15

TP (g/L)

65–82

58.38 ± 2.80

56.00 ± 2.06

57.19 ± 2.00

58.07 ± 2.63

62.59 ± 2.09

60.56 ± 3.18

61.45 ± 3.78

61.07 ± 2.18

ALB (g/L)

27–55

33.76 ± 1.55

32.98 ± 1.29

33.26 ± 0.69

33.51 ± 1.10

36.74 ± 1.34

36.10 ± 2.14

36.16 ± 2.12

36.37 ± 1.72

GLOB (g/L)

18–30

24.62 ± 1.47

23.02 ± 0.92

23.93 ± 1.76

24.56 ± 1.62

25.85 ± 1.36

24.46 ± 1.46

25.29 ± 1.85

24.70 ± 0.66

ALT (U/L)

5–40

34.90 ± 3.51

33.60 ± 3.78

33.40 ± 4.12

32.70 ± 3.13

26.20 ± 4.02

35.33 ± 24.88

25.90 ± 4.72

24.00 ± 2.87

AST (U/L)

100–140

124.30 ± 20.66

136.90 ± 29.06

133.70 ± 17.61

154.70 ± 38.69

124.50 ± 28.38

138.33 ± 77.26

117.20 ± 28.18

126.00 ± 18.48

ALP (U/L)

104–338

181.60 ± 39.16

172.50 ± 25.31

156.00 ± 33.54

171.50 ± 29.44

84.60 ± 16.00

83.00 ± 17.90

86.80 ± 31.85

83.80 ± 26.81

GGT (U/L)

< 0.79

0.30 ± 0.48

0.20 ± 0.42

0.10 ± 0.32

0.40 ± 0.52

0.40 ± 0.52

0.56 ± 0.53

0.10 ± 0.32

0.50 ± 0.53

UREA (mmol/L)

2.7–7.0

5.28 ± 0.62

5.18 ± 0.47

5.12 ± 0.55

4.92 ± 0.60

5.98 ± 1.12

6.03 ± 0.94

6.02 ± 1.03

5.75 ± 0.96

CREA (umol/L)

20–80

19.47 ± 2.19

20.87 ± 1.90

19.34 ± 2.53

19.37 ± 1.43

24.53 ± 4.11

24.21 ± 3.90

24.16 ± 3.18

25.14 ± 2.47

GLU (mmol/L)

7–10.9

7.14 ± 0.78

7.09 ± 0.99

6.14 ± 0.71

7.28 ± 0.98

5.81 ± 0.77

6.19 ± 0.97

6.29 ± 0.71

5.80 ± 0.52

TG (mmol/L)

0.2–1.4

0.31 ± 0.10

0.24 ± 0.08

0.27 ± 0.10

0.29 ± 0.14

0.21 ± 0.06

0.19 ± 0.05

0.20 ± 0.07

0.18 ± 0.08

CHOL (mmol/L)

0.4–1.3

1.43 ± 0.20

1.26 ± 0.22

1.26 ± 0.23

1.36 ± 0.29

1.66 ± 0.31

1.67 ± 0.55

1.60 ± 0.42

1.27 ± 0.34

K⁺ (mmol/L)

3.5–5.0

4.93 ± 0.42

4.89 ± 0.40

4.88 ± 0.41

5.00 ± 0.37

4.34 ± 0.35

4.50 ± 0.28

4.87 ± 0.46*

4.43 ± 0.30

Na⁺ (mmol/L)

135–145

141.30 ± 1.95

141.80 ± 1.32

141.50 ± 1.58

141.60 ± 1.51

140.30 ± 0.67

140.56 ± 1.01

140.80 ± 1.40

140.50 ± 1.18

Cl⁻ (mmol/L)

98–108

103.90 ± 1.66

105.10 ± 0.88

104.60 ± 1.26

104.20 ± 2.66

105.90 ± 0.74

104.78 ± 1.39

105.20 ± 2.35

105.30 ± 1.64

D₅₆

TBIL (umol/L)

0.3–1.2

1.74 ± 0.30

1.64 ± 0.29

1.56 ± 0.30

1.64 ± 0.34

1.74 ± 0.35

1.62 ± 0.28

1.96 ± 0.35

2.34 ± 0.70

n = 5

TP (g/L)

65–82

59.30 ± 2.87

59.64 ± 3.11

60.54 ± 3.26

59.74 ± 1.92

65.60 ± 3.50

63.90 ± 2.03

65.96 ± 2.54

68.84 ± 1.36

ALB (g/L)

27–55

33.30 ± 1.14

33.54 ± 1.43

34.30 ± 1.48

33.74 ± 0.34

38.46 ± 2.82

37.64 ± 0.96

38.56 ± 1.45

39.64 ± 0.59

GLOB (g/L)

18–30

26.00 ± 1.98

26.10 ± 1.75

26.24 ± 1.92

26.00 ± 1.69

27.14 ± 1.51

26.26 ± 1.11

27.40 ± 1.56

29.20 ± 1.37

ALT (U/L)

5–40

46.20 ± 7.33

46.40 ± 7.30

39.00 ± 7.38

38.60 ± 1.14

43.60 ± 11.04

35.20 ± 6.98

32.60 ± 5.46

39.00 ± 4.06

AST (U/L)

100–140

160.80 ± 17.91

159.60 ± 28.92

143.20 ± 27.74

159.40 ± 21.04

136.00 ± 40.63

123.00 ± 15.38

121.20 ± 24.16

144.00 ± 55.19

ALP (U/L)

104–338

114.80 ± 25.01

118.80 ± 22.20

115.00 ± 23.29

99.00 ± 16.84

61.60 ± 13.61

61.40 ± 12.93

61.80 ± 10.03

51.80 ± 12.81

GGT (U/L)

< 0.79

0.20 ± 0.45

0.80 ± 0.45

0.40 ± 0.55

0.60 ± 0.55

0.40 ± 0.55

1.20 ± 0.45

0.80 ± 0.45

0.60 ± 0.55

UREA (mmol/L)

2.7–7.0

7.14 ± 0.67

5.91 ± 0.64*

5.91 ± 0.65*

5.92 ± 0.52*

6.72 ± 0.67

7.38 ± 0.99

6.45 ± 1.31

6.93 ± 1.53

CREA (umol/L)

20–80

23.74 ± 2.59

23.62 ± 2.67

24.60 ± 3.17

25.94 ± 1.23

29.58 ± 4.52

29.82 ± 4.03

28.48 ± 5.00

28.30 ± 5.05

GLU (mmol/L)

7–10.9

8.47 ± 0.91

8.24 ± 2.11

7.67 ± 0.93

7.43 ± 0.50

8.48 ± 1.41

9.14 ± 1.46

8.25 ± 0.61

7.30 ± 0.21

TG (mmol/L)

0.2–1.4

0.35 ± 0.28

0.32 ± 0.11

0.29 ± 0.13

0.24 ± 0.14

0.21 ± 0.06

0.25 ± 0.09

0.27 ± 0.11

0.21 ± 0.04

TCHO (mmol/L)

0.4–1.3

1.34 ± 0.29

1.22 ± 0.40

1.35 ± 0.34

1.34 ± 0.19

1.52 ± 0.20

1.46 ± 0.45

1.49 ± 0.39

1.87 ± 0.45

K⁺ (mmol/L)

3.5–5.0

5.04 ± 0.27

4.88 ± 0.18

4.50 ± 0.10*

4.82 ± 0.27

4.16 ± 0.27

4.10 ± 0.24

4.54 ± 0.21

4.58 ± 0.16

Na⁺ (mmol/L)

135–145

140.80 ± 0.84

141.20 ± 1.30

141.80 ± 1.64

141.60 ± 0.55

140.00 ± 1.00

140.20 ± 0.84

139.80 ± 0.84

140.20 ± 0.84

Cl⁻ (mmol/L)

98–108

103.00 ± 1.22

104.20 ± 1.30

105.20 ± 1.30

104.20 ± 1.10

105.80 ± 0.45

106.20 ± 0.84

106.40 ± 0.89

106.40 ± 1.67