Fig. 2

Effect of esculetin on A) DPPH radical scavenging potentials of esculetin B) Fluorescence intensity of DCFDA, an indicator of ROS level was determined in H9C2 cells. Cells were treated with Esculetin at 25 and 50 25 μM concentrations for 12 h. Each concentration including control (arsenic) tested in triplicate with 75% confluence. Values are presented as the mean ± SEM. * indicates P < 0.05 when esculetin and ascorbic acid treatments compared to control. # indicates P < 0.05 when esculetin, ascorbic acid and control treatments wells compared to Arsenic alone treated well