Fig. 4

Effect of Esculetin on mRNA expression levels against arsenic induced ROS in H9C2 cells. Cells were treated with Esculetin at 25 and 50 25 μM concentrations for 12 h. Each concentration including control (arsenic) tested in triplicate with 75% confluence. The mRNA levels of pro-inflammatory markers (A) TNF-α, (B) IL-6 and (C) NF-κB were measured by using RT-qPCR. Values are presented as the mean ± SEM. * P < 0.05 when compared control with arsenic a P < 0.001 when compared arsenic treatment with esculetin alone. b P < 0.005 when compared arsenic treatment with arsenic + esculetin