Fig. 2

Spontaneous recovery from sunitinib-induced disruption of cardiac sarcomeres (A) Experimental design for analysis of sarcomere morphology following sunitinib washout. (B) Representative immunostaining results for iPSC-CMs following exposure to DMSO or sunitinib (leftmost image) for 72 h; images were obtained 72 h (middle image) and 144 h (rightmost image) after DMSO or sunitinib washout (60×; scale bar: 50 μm). (C) Quantitative analysis of sarcomere morphology via high content image analysis. Data represent the percent change in the HSI of iPSC-CMs relative to the control and are expressed as the mean ratios ± SD. Approximately 200 cells were analyzed in each well, one well was used for each data point, and five or six wells were used as the data for one group (n = 5–6 wells/group). **: p < 0.005, ***: p < 0.0005 versus control group using Shirley-Williams’ multiple comparison test (0 h after washout). ***: p < 0.0005 versus control group using Williams’ multiple comparison test (72 h after washout)