Fig. 3

Nuclear localization of YAP1 in iPSC-CMs 72 h after stimulation with sunitinib and 72 h or 144 h after drug washout (A) Typical immunostaining results for iPSC-CMs following exposure to DMSO (control) or 3 μM sunitinib for 72 h. Nuclei are shown in blue, and nuclear localization of YAP1 is shown in green (white arrows) (4×; scale bar: 100 μm). (B) Quantitative analysis of the extent of nuclear localization of YAP1 via high-content image analysis. Data represent the percent change in the degree of nuclear localization of YAP1 in iPSC-CMs relative to the control and are expressed as mean ratios ± SD. Approximately 4000 cells were analyzed in each well, one well was used for each data point, and six wells were used as the data for one group (n = 6 wells/group). *: p < 0.025, **: p < 0.005 versus control groups using Shirley-Williams’ multiple comparison test. (C) Representative immunostaining results for iPSC-CMs obtained 144 h after washout of 3 μM sunitinib (4×; scale bar: 100 μm). (D) Quantitative analysis of the degree of nuclear localization of YAP1 72 h or 144 h after washout of 3 μM sunitinib via high-content image analysis. Data are mean ratios ± SD. Approximately 4000 cells were analyzed in each well, one well was used for each data point, and five or six wells were used as the data for one group (n = 5–6 wells/group). *: p < 0.05 versus control group using Wilcoxon rank sum test