Fig. 5

Narciclasine mediates autophagy-dependent apoptosis of gastric cancer cells. A-C, Flow cytometry was used to detect the apoptosis rate of gastric cancer cells treated with narciclasine combined with the autophagy inhibitor 3-MA (5 mM) for 24 h. D-E, The MMT assay was used to detect the cell viability of gastric cancer cells treated with narciclasine combined with the autophagy inhibitor 3-MA (5 mM) for 24 h. F-H, Western blotting was used to detect the effects of narciclasine combined with the autophagy inhibitor 3-MA (5 mM) on the expression of apoptotic proteins Bcl-2, Bax, cleaved-PARP, cleaved-caspase-3, 8, 9 and cyto-c in gastric cancer cells after 24 h. Data are shown as mean ± SD. NCS: narciclasine; 3-MA: 3-methyladenine